Genetically Encoded pH Sensor for Tracking Surface Proteins through Endocytosis
Identifieur interne : 001397 ( Main/Exploration ); précédent : 001396; suivant : 001398Genetically Encoded pH Sensor for Tracking Surface Proteins through Endocytosis
Auteurs : Anmol Grover [États-Unis] ; Brigitte F. Schmidt [États-Unis] ; Russell D. Salter [États-Unis] ; Simon C. Watkins [États-Unis] ; Alan S. Waggoner [États-Unis] ; Marcel P. Bruchez [États-Unis]Source :
- Angewandte Chemie International Edition [ 1433-7851 ] ; 2012-05-14.
English descriptors
- Teeft :
- Angewandte chemie, Biological sciences, Biosensor, Carrier control, Cell biol, Cell surface proteins, Chem, Dendritic cells, Donor cells, Endocytic, Endocytic compartments, Energy transfer, Green emission, Imaging, Molecular biosensor, Plasma membrane, Receptor, Receptor trafficking, Recipient cell, Recipient cells, Significant change, Spectral overlap, Surface proteins, Surface selection, Trafficking, Verlag gmbh, Vesicle, Weinheim angew.
Abstract
Traffic cam: A tandem dye prepared from a FRET acceptor and a fluorogenic donor functions as a cell surface ratiometric pH indicator, which upon internalization serves to follow protein trafficking during endocytosis. This sensor was used to analyze agonist‐dependent internalization of β2‐adrenergic receptors (see scheme). It was also used as a surrogate antigen to reveal direct surface‐to‐endosome antigen transfer between dendritic cells (not shown).
Url:
DOI: 10.1002/anie.201108107
Affiliations:
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This sensor was used to analyze agonist‐dependent internalization of β2‐adrenergic receptors (see scheme). It was also used as a surrogate antigen to reveal direct surface‐to‐endosome antigen transfer between dendritic cells (not shown).</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Pennsylvanie</li></region><settlement><li>Pittsburgh</li></settlement><orgName><li>Université Carnegie-Mellon</li></orgName></list><tree><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Grover, Anmol" sort="Grover, Anmol" uniqKey="Grover A" first="Anmol" last="Grover">Anmol Grover</name></region><name sortKey="Bruchez, Marcel P" sort="Bruchez, Marcel P" uniqKey="Bruchez M" first="Marcel P." last="Bruchez">Marcel P. Bruchez</name><name sortKey="Bruchez, Marcel P" sort="Bruchez, Marcel P" uniqKey="Bruchez M" first="Marcel P." last="Bruchez">Marcel P. Bruchez</name><name sortKey="Bruchez, Marcel P" sort="Bruchez, Marcel P" uniqKey="Bruchez M" first="Marcel P." last="Bruchez">Marcel P. Bruchez</name><name sortKey="Salter, Russell D" sort="Salter, Russell D" uniqKey="Salter R" first="Russell D." last="Salter">Russell D. Salter</name><name sortKey="Schmidt, Brigitte F" sort="Schmidt, Brigitte F" uniqKey="Schmidt B" first="Brigitte F." last="Schmidt">Brigitte F. Schmidt</name><name sortKey="Waggoner, Alan S" sort="Waggoner, Alan S" uniqKey="Waggoner A" first="Alan S." last="Waggoner">Alan S. Waggoner</name><name sortKey="Watkins, Simon C" sort="Watkins, Simon C" uniqKey="Watkins S" first="Simon C." last="Watkins">Simon C. Watkins</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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